Association between serum levels of interleukin-12, Chemokine cytokines ligand 1 and occurrence of Type 2 diabetes mellitus complicated with pulmonary tuberculosis

doi:10.3969/j.issn.1000-6621.2017.12.005

Abstract

Abstract: Objective To investigate the relationship between serum levels of interleukin-12 (IL-12), Chemokine cytokines ligand 1 (CCL1) and type 2 diabetes mellitus complicated with tuberculosis (T2DM-PTB). Methods From September 2014 to June 2015 in the Infectious Hospital of Heilongjiang Province, 40 cases of T2DM-PTB patients (group T2DM-PTB), 40 cases of T2DM patients (group T2DM), and 40 healthy people (control group) were selected as the research objects. The demographic characteristics of the subjects were collected. In addition, 3 to 4 ml venous blood was collected in the early morning. The serum levels of IL-12 and CCL1 were mea-sured by enzyme-linked immunosorbent assay and compared among groups. Results The serum concentrations of IL-12 in the control group, T2DM group and T2DM-PTB group were (9.87±2.75) μg/L, (11.25±1.99) μg/L and (13.33±3.67) μg/L, respectively. The difference among the three groups was significant (F=4.46, P<0.01). The serum levels of CCL1 in the control group, T2DM group and T2DM-PTB group were (158.03±29.21) μg/L, (245.02±33.04) μg/L and (381.60±29.99) μg/L, respectively. The difference among the three groups was significant (F=267.90, P<0.01). The serum levels of IL-12 and CCL1 in the T2DM and T2DM-PTB groups were significantly higher than that in the healthy control group, and the serum levels of IL-12 and CCL1 in the T2DM-PTB group was significantly higher compared with the T2DM group (P<0.05). Conclusion The serum levels of IL-12 and CCL1 in patients with T2DM-PTB were significantly increased, suggesting their roles in the pathogenesis of T2DM-PTB.

Key words: Diabetes mellitus, type 2, Tuberculosis, pulmonary, Comorbidity, Interleukin-12, Chemokine CCL1, Data interpretation, statistical

CHEN Hai-jun, LI Yu-ze, LIU Yu-qin, CHEN Xiang-li, YU Tian-jia, LYU He, JIANG Hui, LI Dian-zhong. Association between serum levels of interleukin-12, Chemokine cytokines ligand 1 and occurrence of Type 2 diabetes mellitus complicated with pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2017, 39(12): 1278-1281. doi: 10.3969/j.issn.1000-6621.2017.12.005

References

[1] Hernandez-Pando R, Orozco H, Aguilar D. Factors that deregulate the protective immune response in tuberculosis. Arch Immunol Ther Exp (Warsz), 2009, 57(5): 355-367.

[2] Dobler CC, Flack JR, Marks GB. Risk of tuberculosis among people with diabetes mellitus: an Australian nationwide cohort Study. BMJ Open, 2012, 2(1): e000666.

[3] Wang Q, Han X, Ma A, et al. Screening and intervention of diabetes mellitus in patients with pulmonary tuberculosis in poverty zones in China: rationale and study design. Diabetes Res Clin Pract, 2012, 96(3): 385-391.

[4] 钱荣立. 关于糖尿病的新诊断标准与分型. 中国糖尿病杂志, 2000, 8(1): 5-6.

[5] 中华人民共和国卫生部. 肺结核诊断标准(WS288-2008). 北京: 中华人民共和国卫生部, 2008.

[6] Bafica A, Scanga CA, Feng CG, et al. TLR9 regulates Th1 responses and cooperates with TLR2 in mediating optimal resistance to Mycobacterium tuberculosis. J Exp Med, 2005, 202(12): 1715-1724.

[7] Davila S, Hibberd ML, Hari Dass R, et al. Genetic association and expression studies indicate a role of toll-like receptor 8 in pulmonary tuberculosis. PLoS Genet, 2008, 4(10): e1000218.

[8] Songane M, Kleinnijenhuis J, Netea MG, et al. The role of autophagy in host defence against Mycobacterium tuberculosis infection. Tuberculosis (Edinb), 2012, 92(5): 388-396.

[9] Miller MD, Krangel MS. The human cytokine I-309 is a mono-cyte chemoattractant. Proc Natl Acad Sci U S A, 1992, 89(7): 2950-2954.

[10] Tiffany HL, Lautens LL, Gao JL, et al. Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309. J Exp Med, 1997, 186(1): 165-170.

[11] Pickup JC, Mattock MB, Chusney GD, et al. NIDDM as a disease of the innate immune system: association of acute-phase reactants and interleukin-6 with metabolic syndrome X. Diabetologia, 1997, 40(11): 1286-1292.

[12] Pompei L, Jang S, Zamlynny B, et al. Disparity in IL-12 release in dendritic cells and macrophages in response to Mycobacterium tuberculosis is due to use of distinct TLRs. J Immunol, 2007, 178(8): 5192-5199.

[13] Akira S. Toll-like receptor signaling. J Biol Chem, 2003, 278(40): 38105-38108.

[14] Yeh SH, Chuang H, Lin LW, et al. Regular Tai Chi Chuan exercise improves T cell helper function of patients with type 2 diabetes mellitus with an increase in T-bet transcription factor and IL-12 production. Br J Sports Med, 2009, 43(11): 845-850.

[15] Kamei N, Tobe K, Suzuki R, et al. Overexpression of monocyte chemoattractant protein-1 in adipose tissues causes macrophage recruitment and insulin resistance. J Biol Chem, 2006, 281(36): 26602-26614.

[16] Nakajima K, Tanaka Y, Nomiyama T, et al. Chemokine receptor genotype is associated with diabetic nephropathy in Japanese with type 2 diabetes. Diabetes, 2002, 51(1): 238-242.

[17] Hattori Y, Suzuki M, Hattori S, et al. Vascular smooth muscle cell activation by glycated albumin (Amadori adducts). Hypertension, 2002, 39(1): 22-28.